Q) Explain the effect of pH on the rate of an enzyme-catalyzed reaction
Main points | |
Key idea: | Every enzyme has an optimal pH |
·Ref to enz functioning most efficiently + ref to max rate of rx | |
Key idea: | Deviation from the optimal pH denatures the enzyme |
·Ref to decreases in biological activity of enz ·Reason: ionic & H bonds maintaining specific shape of protein mol including its active site (AS) disrupted ·AS of enz no longer complementary to substrate ·ES complex cannot form | |
Key idea: | Changes in pH affects electrostatic bonds between substrate and active site of enzyme molecule during formation of ES complex |
·Ref to changes in pH can also affect R gps of key aa at AS ·Hence, may affect ionisation of key aa at AS, affect electrostatic binding b/w them, ES complex cannot form | |
Comments: (i) Visualize the answer with the aid of a labelled diagram. (ii) Verbalize the answer + write down the main points w/o referring to the answer (use abbreviations) (iii) Read again within the next 24h & once more within 72h (do not spend more than 10min) | |
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