Q) Explain the effect of temperature on the rate of an enzyme-catalyzed reaction
Main points | |
Key idea: | Enzyme is inactive at low temperature |
·Increasing temp increase kinetic energy of both substrate & enz mol ·Result: higher freq of successful collision b/w enz & substrate mol, form enzyme-substrate (ES) complexes ·Ref to rate of increase doubles /10oC rise in temp (i.e. temp coefficient, Q10 = 2) ·Rate of rx increases until optimum temp of enz reached | |
Key idea: | Enzyme denatures beyond optimum temperature |
·Ref to drastic decrease in rate of rx ·Reason: increase in kinetic energy of enz mol disrupts H & ionic bonds that maintain specific conformation of its active site (AS) ·Result: AS no longer complementary to its substrate; substrate cannot fit into AS to form ES complex | |
Comments: (i) Visualize the answer with the aid of a labelled diagram. (ii) Verbalize the answer + write down the main points w/o referring to the answer (use abbreviations) (iii) Read again within the next 24h & once more within 72h (do not spend more than 10min) (iv) Explain how the other variables (e.g. pH, enzyme and substrate concentrations) affect the rate of enzyme-catalyzed reaction | |
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