Saturday, October 15, 2011

Topic Respiration / Photosynthesis Essay - Main Points!

Q) Distinguish Calvin cycle and Krebs cycle
Main points
Key idea:
Site of occurrence
Calvin cycle: Occurs at stroma of chloroplast 
Krebs cycle: Occurs at mitochondrial matrix
Key idea:
Starting material
Calvin cycle: Ribulose bisphosphate (RuBP) ; eventually regenerated
Krebs cycle: Oxaloacetate ; eventually regenerated
Key idea:
Substrate
Calvin cycle: CO2 used to convert RuBP to an unstable 6C compound that breaks down to form glycerate-3-phosphate + ref to carbon fixation
Krebs cycle: Acetyl-CoA combines w oxaloacetate, to form citrate
Key idea:
Role of CO2
Calvin cycle: CO2 as substrate
Krebs cycle: CO2 released due to decarboxylation rx
Key idea:
Role of O2

Calvin cycle: Not required
Krebs cycle: Occurs only when O2 present
Key idea:
Electron carriers
Calvin cycle: NADPH as e donors ; to reduce glycerate-3-phosphate to glyceraldehyde-3-phosphate
Krebs cycle: NAD+ and FAD as e acceptors ; to oxidize intermediates of cycle
Key idea:
ATP
Calvin cycle: Requires energy throu hydrolysis of ATP
Krebs cycle: ATP syn throu substrate level phosphorylation
Key idea:
Products
Calvin cycle: For every 3 mol of CO2 that enter cycle,1 glyceraldehyde-3-phosphate is made
Krebs cycle: Gives rise to 2 ATP, 6 NADH, 2 FADH2 per glucose molecule*
Key idea:
Nature of the process
Calvin cycle: Anabolic ; syn of hexose phosphates that give rise to starch
Krebs cycle: Catabolic ; oxidation of acetyl-CoA throu a series of decarboxylation +  dehydrogenation rx
Comments:
(i) Visualize the answer with the aid of a labelled diagram.
(ii) Verbalize the answer + write down the main points w/o referring to the answer (use abbreviations)
(iii) Read again within the next 24h & once more within 72h (do not spend more than 10min)
(iv) Glyceraldehyde-3-phosphate is a triose phosphate
(v) Clarification of worked solution – Each rotation of the Krebs cycle gives rise to 1 ATP, 3 NADH, 1 FADH2. For every glucose oxidized, the cycle rotates twice.


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