Tuesday, September 20, 2011

Topic Cell Organelle Essay - Main Points!

Q) Describe rough endoplasmic reticulum, mitochondria and ribosomes in terms of their structural features
Main points
Key idea:
Structural features of rough endoplasmic reticulum (rER)
· Complex network of membranous tubules + sacs (cisternae)
· Ribosomes attached to outer surf of membrane
· which is continous w outer membr of nucleus
Key idea:
Structural features of mitochondria
· Cylindrical or rod-shaped
· Double membrane-bound: outer + inner membr sep by inter-membrane space 
· (i) outer membrane - smooth cont layer + (ii) inner membrane - folded inward, form cristae, project into semi-fluid matrix
· Matrix contains circular DNA + 70S ribo.
· Stalked particles containing ATP synthase (or ATPase) embedded w/n inner membr, for ATP syn
Key idea:
Structural features of ribosomes
· Made up of proteins + ribosomal RNA (rRNA)
· One small + one large subunit (contains peptidyl transferase)
· Euk cell - 40S & 60S subunits ; Prok cell - 30S & 50S subunits
· Exist suspended in cytosol or attached to outer surf of ER
· Has specific sites : (i) Aminoacyl-tRNA binding site (A site) which bind to tRNA w aa
(ii) Peptidyl-tRNA binding site (P site) which bind to tRNA w growing polypep
Comments:
(i) Visualize the answer with the aid of labelled pictures of the organelles (refer to URL provided)
(ii) Verbalize the answer + write down the main points w/o referring to the answer (use abbreviations)
(iii) Read again within the next 24h & once more within 72h (do not spend more than 10min)
(iv) Ribosomes also contains the E site where the uncharged tRNA exits

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