Tuesday, November 1, 2011

Topic: Respiration/Photosyn Essay - Main Points!

Q) Distinguish photophosphorylation and oxidative phosphorylation
Main points
Key idea:
Location
Photophosphorylation
· occurs at thylakoid membr (of chloroplast, plant cells)
Oxidative phosphorylation
· occurs at inner mitochondrial membr (of mitochondria, all euk cells)
Key idea:
Energy conversion
Photophosphorylation
· Light energy converted to chemical energy (in the form of ATP)
Oxidative phosphorylation
· Chemical energy fr glucose converted to chemical energy (in the form of ATP)
Key idea:
Source of energy for ATP synthesis
Photophosphorylation
· Originates fr light
Oxidative phosphorylation
· Originates fr oxidation of glucose
Key idea:
Involvement of light
Photophosphorylation
· Light energy absorbed by photosyn pigments (of light harvesting complexes)
· Excitation & displacement of energized e fr pri pigments (of rx centres of PSI & II)
· Also required for photolysis of H2O, to generate e
· Ref to replenish e lost fr PS II (during non-cyclic photophosphorylation)
Oxidative phosphorylation
· Light not required
Key idea:
Coenzymes
Photophosphorylation
· NADP reduced to NADPH during light dep
· NADPH required for reduction of glycerate-3-phosphate to triose phosphate (TP)
· Some TP used to syn biomolecules (e.g. starch) + rest of TP to regenerate ribulose bisphosphate (RuBP)
Oxidative phosphorylation
· NAD & FAD reduced to NADH & FADH2
· which carry energized e to electron transport chain (ETC), for ATP syn
Key idea:
Source of electrons
Photophosphorylation
· H2O (non-cyclic photophosphorylation)
· PSI (cyclic photophosphorylation)
Oxidative phosphorylation
· NADH + FADH2 donate e to ETC
Key idea:
Oxygen
Photophosphorylation
· not required
· O2 produced as byproduct during photolysis of H2O
· NADP+ - last e acceptor of light dep rx
Oxidative phosphorylation
· O2 - last e acceptor of ETC, hence required for continuous flow of e throu ETC + subseq ATP syn
Key idea:
Water
Photophosphorylation
· Source of e in non-cyclic photophosphorylation (light dep rx)
Oxidative phosphorylation  
· H2O – byproduct, formed when O2 combines with e & H+
Comments:
(i) Visualize the answer with the aid of a labelled diagram.
(ii) Verbalize the answer + write down the main points w/o referring to the answer (use abbreviations)
(iii) Read again within the next 24h & once more within 72h (do not spend more than 10min)

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